03328nas a2200313 4500008004100000245010800041210006900149300001400218490000800232520226700240653002202507653001402529653002202543653002302565653002402588653001402612653002602626653001402652653002202666100002902688700002402717700002302741700002202764700002202786700002302808700002602831700002002857856013702877 2016 eng d00aMorphometrics and molecular phylogenetics of Angraecum section Dolabrifolia (Orchidaceae, Angraecinae).0 aMorphometrics and molecular phylogenetics of Angraecum section D a1027-10450 v3023 a

Background and aims – Recent molecular studies suggest the polyphyly of Angraecum and the
unnaturalness of some of its sections, as exemplified by sect. Pectinaria, which has species in two wellseparated
clades, one in Madagascar and the other in continental Africa. However, species delimitation
among the five continental African members remained problematic due to morphological variability. In
preparation for the taxonomic revision of this group, we used morphological and molecular data to re-assess
the circumscription of each species, and to evaluate their monophyly and relationships to one another.
Methods – A total of 59 alcohol-preserved specimens were used to perform multivariate analyses on 37
morphological characters. DNA sequences from one nuclear (ITS-1) and five plastid regions (matK, rps16,
trnL–F, trnC–petN and ycf1) were analyzed using Parsimony and Bayesian methods.
Key results – The morphometric study revealed five distinct morphospecies that correspond to the concepts
of the currently recognized species. Angraecum doratophyllum and A. subulatum are the most distinct
morphologically, whereas A. atlanticum, A. gabonense and A. pungens are most similar to one another.
Phylogenetic analyses using a combined data set of the six markers yielded highly resolved, congruent
trees with strong branch support. The polyphyly of A. sect. Pectinaria is confirmed, with continental
African members appearing to be most closely related to sect. Dolabrifolia, found exclusively in Africa.
The multiple accessions of A. doratophyllum, A. gabonense, A. pungens and A. subulatum each formed a
well-supported clade. Parsimony and Bayesian analyses placed A. atlanticum and A. pungens in a subclade
within which samples of A. pungens were nested but those of A. atlanticum formed a grade. These two
species can be easily distinguished morphologically by leaf dimensions and flower length, but broader
sampling in continental Africa is needed to test whether individuals recognized as A. atlanticum might
represent two distinct taxa.
 

10aAngraecoid orchid10aAngraecum10aBayesian analyses10acontinental Africa10amolecular phylogeny10amonophyly10amorphometric analyses10aparsimony10asect. Pectinaria.1 aSimo-Droissart, Murielle1 aSonké, Bonaventure1 aDroissart, Vincent1 aMicheneau, Claire1 aLowry, Porter, P.1 aHardy, Olivier, J.1 aPlunkett, Gregory, M.1 aStévart, Tariq uhttps://rubiaceae-africa.myspecies.info/content/morphometrics-and-molecular-phylogenetics-angraecum-section-dolabrifolia-orchidaceae