02356nas a2200193 4500008004100000245012200041210006900163300001200232490000800244520161100252100001801863700002701881700002401908700002101932700002301953700002301976700002001999856014302019 2016 eng d00aPhylogenetics of tribe Sabiceeae (Ixoroideae, Rubiaceae) revisited, with a new subgeneric classification for Sabicea.0 aPhylogenetics of tribe Sabiceeae Ixoroideae Rubiaceae revisited  a551-5800 v1823 a

Tribe Sabiceeae (Ixoroideae, Rubiaceae) has undergone recent taxonomical changes with the incorporation of the
related genera Ecpoma, Pseudosabicea and Stipularia into the type genus Sabicea. We use phylogenetic analysis
and morphological data to verify the relationships among members of the tribe, including the most
comprehensive taxon sampling of the tribe to date with 74 of 145 species. Sequence data from the nuclear
internal transcribed spacer (ITS) and three plastid markers (petD, rps16, trnT–F) were used to infer relationships
among the members of the tribe. Individual analyses using maximum likelihood, parsimony and Bayesian
approaches reveal several supported clades: the former genus Stipularia is resolved as a monophyletic unit, but
Ecpoma is monophyletic only if Sabicea urbaniana and Sabicea xanthotricha are included (corresponding to
Sabicea subgenus Stipulariopsis sensu Wernham). Pseudosabicea is biphyletic, with one clade corresponding to
section Anisophyllae of Halle (1964) and the other one to the other sections (Floribundae and Sphaericae) of the
genus. Eleven morphological characteristics were recorded for all species studied and seven have been mapped
onto the phylogenetic tree to study their evolution in the group and assess their value for the classification of
Sabicea s.l. Finally, our study shows that a combination of diagnostic characteristics should be used to
differentiate each group and we propose to recognise four subgenera in Sabicea.

1 aZemagho, Lise1 aLiede-Schumann, Sigrid1 aSonké, Bonaventure1 aJanssens, Steven1 aLachenaud, Olivier1 aVerstraete, Brecht1 aDessein, Steven uhttps://rubiaceae-africa.myspecies.info/content/phylogenetics-tribe-sabiceeae-ixoroideae-rubiaceae-revisited-new-subgeneric-classification